Thrombin cleaves

Calculate the actual molecular weight of the three bands by doing a ratio calculation, based on the observed MALDI MW of the apomyoglobin standard and the actual MW of the apomyoglobin standard. Ratio = observed value/actual value.

Affinity chromatography protein Purification Guidelines for the Affinity Purification Lab Report (lab report 4, to be written individually) 1) Estimate the molecular weights (using Rf values) of the three prominent bands you see in your thrombin digest lane. 2) You have been provided with two results of a MALDI-TOF analysis (pp. 102-103 above). a) Calculate […]

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