protein X hybrid

What does the GAL4 protein normally do in Saccharomyces cerevisiae (Brewer’s yeast)? What are the attributes of GAL4 that enable it to be used in the yeast two hybrid system?

Journal Club 3: Fields & Song, Nature, 1989 The authors state that “protein-protein interactions between two proteins have generally been studied using biochemical techniques such as cross-linking, co-immunoprecipitation and co-fractionation by chromatography.” Briefly describe one of these techniques. What does the GAL4 protein normally do in Saccharomyces cerevisiae (Brewer’s yeast)? What are the attributes of […]

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