Guanidine thiocyanate

What is Vmax and why does the activity plot level off at high substrate concentrations? Provide the chemical structure and 1-2 sentences about each of these reagents as they relate to protein structure, function and/or stability.

CHEM322 Foundations of Chemical Biology Laboratory β-Lactamase: Protein Kinetics and Stability, Week 3  You have now characterized the purity and relative activity of the protein that you purified. This week you will utilize your pooled protein samples to examine two things:   Kinetic parameters for the purified enzyme using a continuous enzyme activity assay. Review the […]

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