GMO and PCR Lab Report
Detection of Genetically Modified (GM) Foods by PCR
This lab takes two lab sessions and has different parts, – Genomic DNA extraction from foods, DNA amplification by PCR and finally the DNA agrose electrophoresis of PCR products. You will do the first two tasks in today’s lab and carry out the electrophoresis part in Lab#11
Detection of Genetically Modified (GM) Foods by PCR
1.How can you test a food to find out if it contains material derived from a genetically modified organism(GMO)?
2.Why do you also perform analysis on food that is known to be a non-GMO control?
3.What chemicals and molecules are needed for PCR, and what is the function of each component?
4.Why are you performing two PCR reactions on each DNA sample?
5.Explain why DNA fragments separate according to size in an electrophoresis gel.
6.Attach your gel picture and analyze each band.
7.Did your test food generate a 200 bp band with GMO primer (lane 4)? What does this tell you about the GMO status of your food?
