Determine the blood types of the four volunteers. Determine whose blood can be donated to the patient and explain why the others can not be used.

Clinical scenario
A patient is being prepped for immediate surgery. Blood will be needed for a transfusion. The patient’s blood type is B+. However, the blood bank is out of compatible donor blood. Four nurses volunteer to donate blood so the blood bank has to determine whose blood can be used.

Introduction:
General background about blood types (ABO and Rh) noting antigens and antibodies for each
Brief explanation of how the testing works

Materials used:
4 3-well blood typing trays
4 volunteer “blood” samples (Brown, Smith, Jones, Green)
Anti-A antibody
Anti-B antibody
Anti-Rh antibody
12 stirring sticks

Procedure:
For each volunteer “blood” sample, put 2 drops of blood into three wells of one blood typing tray
Put two drops of Anti-A, Anti-B, and Anti-Rh antibodies in to the corresponding wells
Gently stir each well with a separate stick to prevent cross contamination
Agglutination (clumping) signifies a positive result, no agglutination is negative

Data:
Anti-A Anti-B Anti-Rh
Jones Neg Pos Neg
Smith Pos Neg Pos
Green Pos Pos Pos
Brown Neg Neg Neg

Conclusion:
Determine the blood types of the four volunteers.
Determine whose blood can be donated to the patient and explain why the others can not be used.

Determine the blood types of the four volunteers. Determine whose blood can be donated to the patient and explain why the others can not be used.
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