Use standard curve to determine concentrations of Dopachrome in Tyrosinase reactions to determine rate of enzyme activity.

Spectrophotometric Determination of enzyme activity (Tyrosine)

Talk to your paired group, decide who is doing Russet and who is
doing either Red potato or Yukon gold.

Each group gets 1 of their potato.

Cut off a small piece for your paired group to visualize and give it to
them.

Purpose

What are we doing today in lab? What are we comparing and how?

What are we testing here, what do you expect to happen and why do
you expect it?

Standard Curve

Use standard curve to determine concentrations of Dopachrome in Tyrosinase reactions to determine rate of enzyme activity.

1st spin done

Do you keep the supernatant or the pellet?
Where is your enzyme?
What about for the second spin?